Figure 1.

rho2Δ and pck2 mutant cells have similar cell wall defects. Shown are electron microscopy images of fission yeast cells expressing different levels of Rho2p. HM123 (wild-type) cells carrying pREP1 (a) or pREP1-rho2 (b); KN-1 (rho2Δ) (c), and TP179–1A (sts6–8=pck2) mutant cells (d) were grown at 30°C for 16 h in EMM liquid medium without thiamine before fixation. Arrows indicate membranous structure. N, nucleus, Mt, mitochondria. Twofold-magnified images are shown in insets. More than 30 cells of each sample were observed, and all display a similar phenotype. Bar, 1 μm.