Figure 2.

Hypersensitivity to cell wall inhibitors of different S. pombe strains. Wild-type (HM123), rho2Δ (KN-1), Pnmt81-pck1 (MA20), Pnmt81-pck2 (MA21), Pnmt81-pck1 rho2Δ (TMC110), and Pnmt81-pck2 rho2Δ (TMC111) cells were grown at 28°C in EMM with thiamine, spotted on EMM with thiamine plates at 4 × 10⁶ and 10⁶ cells/ml, and incubated 2–3 d at 28°C. Drug concentrations were as follows: Calcofluor (CF) (0.5 mg/ml), Papulacandin B (Pap) (2 and 4 μg/ml); and Echinocandin (Ech) (1, 2, and 4 μg/ml).