Figure 3.

Hypersensitivity of rho2Δ cells to glucanases is suppressed by pck2+ overexpression. S. pombe wild-type (⋄) and rho2Δ (KN-1) cells transformed with the plasmids pREP1 (□), pREP1-rho2 (○), and pREP1-pck2 (▵) were grown at 32°C in minimal medium with 5 μM thiamine for 20 h and resuspended in TE buffer with 20 mg/ml β-glucanase complex (Zymolyase 100T). Cell lysis was monitored by measuring optical density at 600 nm as a function of time. Values were calculated from at least four independent experiments. Means and SDs are shown.