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rho2+ overexpression does not cause any effects in the absence of Pck2p. (A) Growth of wild-type (HM123), pck1Δ (TP134–3B), and pck2Δ (TP169–1C) cells transformed with pREP3X, pREP4X, pREP3X-rho2, or pREP4X-rho2. Cells were grown on EMM plates without thiamine. (B) Localization of F-actin in (a) HM123, (b) TP169–1C, (c) HM123 transformed with pREP3X-rho2, (d) TP169–1C transformed with pREP3X-rho2. Bar, 5 μm.
