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Synthetic lethality of rho2Δ and mok1–664 mutations. (A) Wild-type (HM123), rho2Δ (KN-1), Pnmt81-pck2 (MA21), mok1–664 (DH664), Pnmt81-pck2 rho2Δ (TMC111), mok1–664 rho2Δ (TMC113), and mok1–664 pck2Δ (SKP100) cells were grown in YES medium at 28°C, spotted on YES solid-medium plates, and incubated for 3 d at different temperatures. (B) Phase-contrast micrographs of the same strains grown in YES liquid medium for 20 h at 32°C. Bar, 10 μm.
