Table 2.
Incorporation of radioactivity from 14C-glucose into cell wall polysaccharides of different S. pombe strains grown in minimal medium without thiamine for 16 h
| Strain | Plasmid | Cell wall | Galactomannan | α-Glucan | β-Glucan |
|---|---|---|---|---|---|
| HM123 (wild-type) | 29.2 ± 1.8 | 2.3 ± 0.5 (8) | 9.5 ± 0.8 (33) | 17.4 ± 1.8 (60) | |
| KN-1 (rho2Δ) | 25.6 ± 0.4 | 2.3 ± 0.7 (9) | 7.6 ± 0.2 (30) | 15.7 ± 1.2 (61) | |
| TP179-1A (sts6–8) | 23.3 ± 3.7 | 3.5 ± 1.1 (15) | 6.8 ± 0.7 (29) | 13.0 ± 2.4 (56) | |
| TP169-1C (pck2Δ) | 24.7 ± 0.6 | 4.1 ± 0.9 (17) | 6.6 ± 0.4 (27) | 14.0 ± 1.0 (57) | |
| TP134-3B (pck1Δ) | 24.3 ± 2.1 | 3.9 ± 1.4 (16) | 8.2 ± 1.0 (34) | 12.2 ± 1.3 (50) | |
| HM123 | pREP4X | 29.8 ± 1.6 | 2.1 ± 0.8 (8) | 10.0 ± 0.8 (33) | 17.7 ± 1.5 (59) |
| HM123 | pREP4X-rho2 | 38.9 ± 3.3 | 1.5 ± 0.7 (4) | 17.2 ± 1.6 (44) | 20.1 ± 1.8 (52) |
| TP169-1C | pREP4X-rho2 | 23.3 ± 2.1 | 4.5 ± 1.2 (19) | 6.4 ± 0.8 (27) | 12.4 ± 0.9 (53) |
| TP134-3B | pREP3X-rho2 | 39.8 ± 2.2 | 4.1 ± 1.0 (10) | 16.8 ± 1.2 (42) | 18.9 ± 0.9 (47) |
14C-glucose was added 2 h before harvesting the cells.
Values indicate percentage of total 14C-glucose incorporated and are the mean ± SD calculated from at least four independent experiments. Values in parentheses are the percentage of the corresponding polysaccharide in the cell wall.