Figure 5.

The RXRα ligand, 9-cis -RA, sensitizes T24 bladder cancer cells to troglitazone or 15dPGJ₂-induced cell death. Upper panel, quiescent T24 cells were treated with troglitazone (0.1, 1, 5, 10, 15, and 20 µM) in the presence or absence of 9-cis -RA LG100268 (5 µM) for 24 hours. MTT assay was performed to measure cellular viability. Values represent the percentage of inhibition of control culture (treated with vehicle alone) from five independent experiments. Error bars are standard errors of mean (SEM) (n=20; *P<0.05, **P<0.01). Lower panel, concentration-dependent effects of 15dPQJ₂ on T24 cell viability. Study was performed in the presence or absence of 9-cis -RA (5 µM). Values represent the percentage of control culture (treated with vehicle alone) values from three independent experiments. Error bars are standard errors of mean (SEM) (n=12; *P<0.05, **P<0.01).