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ZNF198-FGFR1 transforms Ba/F3 cells to IL-3 independence. Ba/F3 cells were transfected with pcDNA3.1 or pcDNA3.1/ZNF198-FGFR1. Cells were initially selected for G418 resistance before plating in media lacking IL-3. IL-3-independent cells (pcDNA3.1/ZNF198-FGFR1) or G418-resistant cells (pcDNA3.1) were washed free of IL-3 and plated in the absence of presence of IL-3. Viable cells were counted on each day, and results shown are the average of three independent clones.
