Figure 5.
ZNF198-FGFR1 oligomerises in vitro. (A) pcDNA3.1/ZNF198-FGFR1 (lane 1), pcDNA3.1/ZNF198-FGFR1ΔC-myc (lane 2), or a mixture of the two (lane 3) were in vitro transcribed and translated in the presence of [35S] methionine. (B) One half the reaction mixture was immunoprecipitated by using either an antisera to the C-terminus of human FGFR1 (lanes 1–3) or an anti-myc epitope antibody (lanes 4–6). Total reaction products or immunoprecipitates were separated by SDS/PAGE, the gel fixed, treated with Amplify (Amersham), dried, and exposed to film at -70°C.