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. 1999 Dec;1(6):498–507. doi: 10.1038/sj.neo.7900066

Figure 2.

Figure 2

Microtubule polymerization inhibition by IAABE and BAABE. Compounds were dissolved in G-PEM buffer at 4°C before resuspending the lyophilized tubulin in the wells of a 96-well plate (CytoDYNAMIX™ Screen 01; Cytoskeleton Inc.). Absorbance was measured over time; absorbance is proportional to microtubule content. IC50 values were determined by linear regression analysis of concentration versus percentage inhibition; concentration at 50% inhibition at 60 minutes was defined as the IC50. Concentrations of IAABE (A) were 0 (■), 1.0 (♦), 2.5 (▲), 5.0 (□) and 10 µM (○). Concentrations of BAABE (B) were 0 (■), 10 (♦), 20 (▲), 40 (□) and 80 µM (○).