Figure 4.

Sulindac sulfone does not alter the growth of HCA-7 or HCT-116 xenografts. HCA-7 (A) or HCT-116 (B) cells were grown to 70% confluence and then trypsinized; 5 x 10⁶ cells were injected subcutaneously into the dorsal flank of athymic mice. The mice were divided into treatment and control groups. The initial dose of sulindac sulfone was 10 mg/kg and then increased to 100 mg/kg by week 5. The volume of the tumor was calculated according to the equation (V = [L x W²] x 0.5), where V = volume, L = length, and W = width (insert). HCA-7 cells (5 x 10⁶ cells) were xenograft into nude mice. After 7 weeks of tumor growth intraperitoneal injections with sulindac sulfide were initiated at a dose of 10 mg/kg every other day. Tumor measurements were obtained as described.