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. 2006 Apr 3;7:186. doi: 10.1186/1471-2105-7-186

Table 2.

Ranges of allowed variations of kinetic parameters

Enzyme	Parameter	Reference value	Minimum	Maximum
GLK	V_max	226.452	150	100000
	K_eq	3800	1	100000
	K_m·Glc	0.08	0.005	1
	K_m·G6P	20	0.02	1000
	K_m·ATP	0.15	0.0001	0.3
	K_m·ADP	0.23	0.1	1000

PGI	V_max	339.677	30	100000
	K_eq	0.314	0.002	1000
	K_m·G6P	1.4	0.001	100
	K_m·F6P	0.3	0.001	1000

PFK	V_max	182.903	50	100000
	g_R	5.12	0.5	1000
	L₀	0.66	0.1	50000
	K_m·ATP	0.71	0.001	10
	K_m·F6P	0.1	0.001	10
	K_AMP	0.0995	0.0001	10000
	K_ATP	0.65	0.0001	10000
	K_F26bP	0.000682	0.0001	10000
	K_F16bP	0.111	0.0001	10000

ALD	V_max	322.258	100	100000
	K_eq	0.069	0.0005	10
	K_m·F16P	0.3	0.001	50
	K_m·GAP	2	0.0001	10
	K_m·DHAP	2.4	0.001	100
	K_m·GAPi	10	0.02	1000

G3PDH	V_max	70.15	2	300
	K_eq	4300	0.01	100000
	K_m·DHAP	0.4	0.001	500
	K_m·NADH	0.023	0.001	50
	K_m·NAD	0.93	0.001	1000
	K_m·Gly	1	0.0001	1000

GAPDH	V_max	1184.52	200	100000
	K_eq	0.045	0.005	10
	K_m·GAP	0.21	0.001	2
	K_m·BPG	0.0098	0.0005	1000
	K_m·NAD	0.09	0.0001	5
	K_m·NADH	0.06	0.0005	8

PGK	V_max	1306.45	30	100000
	K_eq	3200	100	100000
	K_m·BPG	0.003	0.0001	10
	K_m·P3G	0.53	0.001	30
	K_m·ADP	0.2	0.002	1000
	K_m·ATP	0.3	0.001	30

PGM	V_max	2525.81	50	100000
	K_eq	0.19	0.001	100
	K_m·P3G	1.2	0.001	300
	K_m·P2G	0.08	0.00002	100

ENO	V_max	365.806	200	100000
	K_eq	6.7	0.01	1000
	K_m·P2G	0.04	0.0001	10
	K_m·PEP	0.5	0.00003	100

PYK	V_max	1088.71	300	100000
	K_eq	6500	0.1	9000
	K_m·PEP	0.14	0.01	9
	K_m·Pyr	21	0.1	1000
	K_m·ADP	0.53	0.01	4
	K_m·ATP	1.5	0.3	100

PDC	V_max	174.194	100	100000
	K_m·Pyr	4.33	0.001	50

ADH	V_max	810	200	100000
	K_eq	6.9·10^-5	10^-8	0.0005
	K_m·Ace	1.11	0.01	1000
	K_m·Et	17	0.001	1000
	K_m·NADH	0.11	0.001	5
	K_m·NADP	0.17	0.001	30