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. 2000 Dec;11(12):4403–4411. doi: 10.1091/mbc.11.12.4403

Figure 6.

Figure 6

Coprecipitation of Ypt1 and Ypt31/32 With GST-Bet3 and TRAPP. (a) GST-Bet3p or GST were expressed in yeast cells and purified using GA. Equal volumes of eluted preps were analyzed by SDS-PAGE followed by western blotting using anti-Ypt1 (top panel) or Ypt31/32 (lower panel) antibodies. Ypt1 and Ypt31/32 proteins coprecipitate only with GST-Bet3p but not with GST alone. (b) Superdex 200 column fractions containing GST-Bet3, high MW (fraction 30), low MW (fractions 46–48), and the GST-Bet3 fraction that was loaded on the column (L), were analyzed by SDS-PAGE followed by western blotting using anti-Ypt1 (top panel), Ypt31/32 (middle panel), or anti-GST (lower panel) antibodies. Ypt1 and Ypt31/32 proteins coprecipitate only with the High MW but not with the Low MW GST-Bet3p.