Figure 3.

Formation of radioactive UDP-glucose and cellulose from [glucose-¹⁴C]sucrose in transformants of A. xylinum. (A) UDP-[¹⁴C]glucose formed from [glucose-¹⁴C]sucrose. •, Transformant with mutant sucrose synthase cDNA; ○, the transformant with wild-type sucrose synthase cDNA; ▵, plasmid-free cells. (B) [¹⁴C]Cellulose formed from [glucose-¹⁴C]sucrose, in which the amount of cellulose is shown as the height of bar due to total radioactive plus nonradioactive cellulose in each fraction, the solid part of which is due to the radioactive cellulose equivalent to specific radioactivity of [¹⁴C]glucose moiety: ■, mutant sucrose synthase cDNA; , wild-type sucrose synthase cDNA; ▨, plasmid-free cells.