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Levels of sucrose synthase activity and its substrates/products in A. xylinum transformants
| Plasmid | Forming activity, nmol per mg per min
|
Content, μmol/ml*
|
|||
|---|---|---|---|---|---|
| UDP-glucose | Sucrose | UDP | Sucrose | UDP-glucose | |
| None | 0 | 0 | 0.20 | 25 | 0.25 |
| pSA-SD (wild type) | 60 | 37 | 0.14 | 20 | 0.31 |
| pSA-SD-S11E (mutant) | 140 | 42 | 0.02 | 18 | 0.41 |
Concentrations of UDP-glucose, UDP, and sucrose are shown per packed cell volume (ml).
