Figure 1.

Generation of Man/GalNAc-4-SO₄ receptor^+/– mice. (a) The Man/GalNAc-4-SO₄ receptor targeting construct. The 5′ and 3′ homologies were derived from exon 2, which encodes the entire cysteine-rich domain of the receptor, and its flanking introns. Exon 2 was replaced with a PGK-neo cassette. (b) DNA from the live progeny of heterozygous matings was genotyped by PCR or Southern blotting. The wild-type locus was detected using primers P1 and P2, which yielded a 421-bp product, and the mutant locus using primers P3 and P4, which yielded a 548-bp product. No Man/GalNAc-4-SO₄ receptor^–/– animals lived to birth. Examples of PCR reactions from M/G4S-R^+/+ and M/G4S-R^+/– mice are shown. Std, oligonucleotide standard.