Figure 4.

Man/GalNAc-4-SO₄ receptor message is reduced in M/G4S-R^+/– mice as compared with M/G4S-R^+/+ mice. Total RNA was prepared from the tissues indicated. Three micrograms of total RNA were used to synthesize first-strand cDNA for each tissue. The amount of Man/GalNAc-4-SO₄ receptor message for identical inputs of total RNA was quantitated by PCR on a LightCycler by comparison to Man/GalNAc-4-SO₄ receptor cDNA of known concentration using the primers specific for exon 7. The values shown represent the mean of two determinations in each case. The duplicates for each determination differed by less than 5%.