Figure 1.

Effect of aldosterone on the expression of prostasin mRNA in M-1 cells. M-1 cells, which were serum deprived for 48 hours, were treated with 1 μM aldosterone and harvested at various time points (0, 6, 12, 24, and 48 hours) for isolation of total RNA. Each lane contains 20 μg of total RNA. The abundance of prostasin mRNA was normalized for β-actin (bottom). Aldosterone induced an increase in prostasin mRNA after 6 hours of stimulation. Maximum induction occurred at 24 hours (1.9-fold ± 0.2-fold increase), and this effect was observed for up to 48 hours. This blot is representative of four separate experiments. Values are mean ± SEM (n = 4). *P < 0.02.