Figure 5.

Effect of aldosterone infusion on prostasin excretion in the urine. Immunoblot showing the effect of aldosterone infusion (100 μg/100 g body weight/day) for 7 days on the abundance of prostasin excreted into rat urine. Urine samples that are equivalent to 80 μg creatinine excretion were TCA precipitated, and urinary prostasin excretion was determined by immunoblotting. Aldosterone infusion resulted in a substantial increase in prostasin excretion into the urine. The effect of aldosterone was time dependent, and maximum induction was observed at day 7 (4.3-fold ± 2.0-fold increase over control). This figure is representative of five separate experiments. Values are mean ± SEM (n = 5). *P < 0.004.