Figure 2.

In vitro PMN function and in vivo response to thioglycollate in the absence of DPPI. (a) Bone marrow–derived PMNs from WT and DPPI^–/– mice were allowed to migrate across a filter in response to 10^–4 M fMLP, 2% zymosan-activated rat serum (ZAS), or 3 μg/ml rhIL-8. The number of cells that migrated was expressed as a percentage of total PMNs. Values represent the mean ± SEM of at least three animals. (b) Superoxide production by PMNs in response to PMA, expressed as nmol of O₂^– generated per 10⁶ PMNs (n > 3 per genotype). The number of PMNs and macrophages recruited in response to intraperitoneal injection of thioglycollate is normal in DPPI^–/– mice at 4 hours (c) 24 hours (d) and 120 hours (e).The slightly lower number of macrophages at 120 hours after thioglycollate injection in the DPPI^–/– mice did not reach statistical significance (n = 4–5 mice per genotype). Mph, macrophages.