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. 1999 Jan 5;96(1):23–28. doi: 10.1073/pnas.96.1.23

Figure 2.

Figure 2

Colocalization of CIR and CBF1 in transfected Vero cells. In indirect immunofluorescence assays, Flag-CBF1 (Left, red) and CIR (Center, green) each show a mixture of diffuse nuclear staining and strongly staining, irregular nuclear speckles. In the merged images (Right), colocalized staining appears yellow. The pattern indicates colocalization between the majority of CBF1 and CIR nuclear speckles. Primary antibodies were mouse anti-Flag mAb and rabbit anti-peptide CIR antibody. Secondary antibodies were rhodamine-conjugated anti-mouse Ig (red) and fluorescein isothiocyanate (FITC)-conjugated anti-rabbit Ig (green).