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. 2002 Feb 15;109(4):481–489. doi: 10.1172/JCI12867

Figure 6.

Figure 6

IMCD cells expressing the C-terminal domain of polycystin-1 form multicellular tubular structures. IMCD cells expressing either CD16.7.PKD1 (PKD4077–4302) or the patient-derived truncation mutation CD16.7.R4227X (PKD4077–4227) were cultured for 5 days in a Matrigel/type I collagen mixture. Single-cell branching by cells expressing CD16.7.PKD1 on culture days 1 and 2 correlated with the formation of branching tubular structures by days 4 and 5. Expression of the CD16.7.R4227X truncation mutant did not support branching on days 1 and 2, and resulted in the formation of multicellular spherical structures by days 4 and 5. The development of lumens in both types of structures began by day 5 of culture. Magnification of images, ×20; representative structures are shown.