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. 2002 Mar 1;109(5):661–670. doi: 10.1172/JCI13572

Figure 2.

Figure 2

CF sol inhibits HMDM ingestion of apoptotic but not IgG-opsonized Jurkat cells in an elastase-dependent manner. (a) HMDMs were cocultured with apoptotic, opsonized, or viable Jurkat cells in the presence or absence of 10% CF sol. The mean phagocytic index as percentage of control ± SEM is shown for three replicates per group. Control mean phagocytic index: 28.6 ± 4.4. *Significantly different from apoptotic Jurkat cells pretreated with media (P < 0.05). (b) HMDMs were cocultured with apoptotic Jurkat cells in the presence of media (Control), 10% CF sol, 10% CF sol plus DMP777 (100 μM), 10% CF sol plus SJ527 (100 μM), or 10% CF sol plus methyl cellulose (MC). The mean phagocytic index as percentage of control ± SEM is shown for three to five replicates per group. Control mean phagocytic index: 52.8 ± 10.2. *Significantly different from control (P < 0.05). (c) HMDMs, apoptotic Jurkat cells, or both were pretreated for 30 minutes with media (Control), 10% CF sol, 10% CF sol plus DMP777 (20 μM), or DMP777 (20 μM) alone prior to coculture. Following pretreatment, coculture was done in the presence of DMP777 (20 μM) to prevent carryover of elastase activity. The mean phagocytic index as percentage of control ± SEM is shown for three replicates per group. Control mean phagocytic index: 51.4 ± 6.0. *Significantly different from control (P < 0.05). **Significantly different from 10% CF sol-pretreated HMDMs (P < 0.05). ***Significantly different from 10% CF sol-pretreated HMDMs and apoptotic Jurkat cells (P < 0.05).