Figure 3.

GA-specific CD4⁺ and CD8⁺ T cell responses are HLA-restricted. CFSE-based proliferation assays were performed on PBMC specimens from five MS patients on daily GA therapy for 3 months in the presence of anti-HLA antibodies or appropriate control Ig. (a) These dot plots show CD4⁺ and CD8⁺ GA-specific proliferative responses from a representative patient (no. MS2) in the presence of indicated antibodies. The two left panels demonstrate the effect of HLA class I blockade (compared with the IgG1 control), whereas the two right panels demonstrate the effect of HLA class II blockade (compared with IgG2a control). CFSE staining is shown on the x axis and CD8 staining on the y axis. The numbers represent the proliferating fraction of CD4⁺ and CD8⁺ T cells. (b) This graph represents mean ΔPF (+ 2 SEM) values of CD4⁺ and CD8⁺ T cell responses to GA from all five patients in the presence of indicated antibodies. (c) The same data are expressed in the form of percent response, where the proliferation in the presence of appropriate control Ig was assigned a 100% value.