Figure 2.

Affinity determination of mouse affinity-purified Ab’s to the peptide. Using several molar concentrations of NH₄SCN, the percentage of affinity-purified antipeptide Ab’s binding to the peptide was determined by ELISA. The antipeptide Ab’s were derived from mice immunized with H. influenzae (HI), N. gonorrhoeae (NG), tetanus toxoid (TeNT), Streptoccocus pneumoniae (SP), Shigella dysenteriae (SD), or C. albicans (CA) at Ab concentrations of 20 μg/ml. Data are presented as percentage of binding in three separate experiments.