Figure 2.

(a and b) Expression of human iNOS mRNA (RT-PCR) and iNOS protein (Western blot) in hearts of iNOS⁺/αMtTA⁺ mice in the presence and absence of DOX (200 μg/ml in drinking water) and nonbinary littermate controls. (c) Calcium-independent (iNOS) and total NOS activities in cardiac extracts of iNOS⁺/αMtTA⁺ mice and nonbinary littermate controls, n = 5. (d and e) β-gal (blue staining), (f and g) iNOS (green immunofluorescence), (h and i) NADPH-diaphorase (dark blue staining), and (j and k) NO-Y (brown staining) of cardiac sections from iNOS⁺/αMtTA⁺ mice in the presence (+) and absence (–) of DOX.