Figure 3.

(a) RPA of the retinal VEGF mRNA levels. VEGF₁₂₀, VEGF₁₆₄, and 18S mRNA is shown. The figure shows protected fragments from nondiabetic animals, diabetic animals treated with control implants, and diabetic animals treated with insulin implants. (b) VEGF/18S mRNA signal ratio quantitation. After normalization to the 18S signal, VEGF mRNA levels were increased 94.4% following the induction of diabetes (15.47 ± 4.6 to 30.07 ± 6.7, n = 4–5 per condition, P < 0.01 vs. nondiabetic animals), and another 70% in the insulin-treated animals (51.1 ± 10.4, n = 3–5, P < 0.005 vs. nondiabetic animals).