Figure 1.

Generation of α_1D-AR–deficient mice. (a) Simplified restriction map around exon 1 of the α_1D-AR gene and structure of the targeting vector. The coding region of the exon is boxed. Neo, PGK-neo cassette; DT, diphtheria toxin-A fragment gene; B, BamHI; E, EcoRI; EV, EcoRV; H, HindIII; Sa, SalI; SI, SacI; SII, SacII. (b) Southern blot analysis of tail DNA. DNA was digested with EcoRV, and the blot was hybridized with the 3′ probe shown in a. The 7-kb band is derived from the wild-type allele (wild) and the 4-kb band from the targeted allele (mutant).