Table 1.
PCR primer sequences used to place cataract orthologs on canine chromosomes using an RH5000 panel
| Gene | GenBank accession number | Primer sequences | Tm (°C) | Product size (bp) |
|---|---|---|---|---|
| Structural protein genes | ||||
| BFSP2 | NM_003571 | F: CCTCTTCATAGTTCCTTGAC | 50.7 | 419 |
| R: CCCTACAGTTCTATGCTTCCAG | 55.8 | |||
| CX46 | NM_021954 | F: CCAGAGAATGCTAATTTGTCCC | 54.9 | 365 |
| R: CAGCACATGCACTTACACAGG | 57.6 | |||
| CX50 | NM_005267 | F: GGGATAACCAGAGGGTAGCAC | 57.8 | 386 |
| R: GGATCTCGAAGTTCTCCTACC | 54.9 | |||
| LIM2 | NM_030657 | F: GGCAACAAGTGCTACCTGC | 56.9 | 353 |
| R: CCCAGTTTCCAACCTAGTTC | 53.0 | |||
| MIP | NM_012064 | F: GGGCACGAGTACGAGCACTG | 61.0 | 308 |
| R: GCCTGCACGCTTCGCAAGC | 63.5 | |||
| SPARC | NM_003118 | F: CTGATCATGTGGCCTTGAGC | 56.2 | 343 |
| R: GGCCTAATCTGATCTGCTAAG | 52.5 | |||
| Crystallin genes | ||||
| CRYBB2 | NM_000496 | F: GGAGAGCAGTTTGTGTTCGAG | 55.9 | 356 |
| R: GTGCAATGTGGCAACCCTTAG | 56.9 | |||
| CRYGA | NM_014617 | F: CCTACGAAGGAATCCCTTTTG | 53.2 | 349 |
| R: GAGTGGAGCTCATTGAGCCG | 58.1 | |||
| CRYGB | NM_005210 | F: GCTCCTGGCATCCAGTGGG | 61.0 | 404 |
| R: CTGCTCCTGCTGCTCTACTC | 57.3 | |||
| CRYGC | NM_020989 | F: CTCTAACCCAGACTCTGAACC | 54.3 | 373 |
| R: GGACCCAGCAGCCCTCCAG | 63.3 | |||
| CRYGD | NM_006891 | F: GACAGCGGCTGCTGGATGC | 62.1 | 381 |
| R: CCAGGACAGGACCTATTGCTG | 57.4 | |||
| CRYGS | NM_017541 | F: CAGCTCATCCCAAGACTGAATG | 55.7 | 390 |
| R: GACACCATCCAGCACCTTACTG | 57.7 | |||
| Transcription factors | ||||
| CHX10 | NM_182894 | F: CGTAAGTTAAGTACCAGAGGG | 52.1 | 390 |
| R: CTAAACCTGTGATTTCTGTGCC | 54.0 | |||
| EYA1 | NM_172060 | F: CTACTGCCCAAACTCATCAC | 53.2 | 386 |
| R: CCCACAGAAAGAATGTAGATG | 50.7 | |||
| FOXE3 | NM_012186 | F: GCAAGTCACTGCAGGGACTG | 58.6 | 342 |
| R: GTTCTTTAGTGGTGCTGGAGG | 55.5 | |||
| HSF4 | NM_001538 | F: GATTCCTGAGCTCTACCATAG | 51.7 | 463 |
| R: GCCAGGGTCTGGTTGAAGC | 58.9 | |||
| MAF | NM_005360 | F: GTTAACAGCACGGAGGTTACAC | 55.9 | 340 |
| R: CCCTTCCACTGCATCTGAGTC | 57.6 | |||
| PAX6 | NM_001604 | F: GCCACATCTTCAGTACAAAG | 49.6 | 300 |
| R: TAGTTCAGGCATTGACTGATG | 50.3 | |||
| PITX3 | NM_005029 | F: CCTCCCTGAACAGGGTGATAG | 57.5 | 304 |
| R: CCGCACCATTGCACACCACG | 63.0 | |||
| SIX5 | NM_175875 | F: GACCCGCAGCTTCTCAAGC | 59.0 | 371 |
| R: CCTTCCTACTGCAAAGTGAGC | 55.8 | |||
| SOX1 | NM_005986 | F: CTCAAGAATGATATCCACTGCTTC | 53.5 | 299 |
| R: GTACATTTCAGAGTCAATGTGGC | 54.1 | |||
The column labeled “Tm” indicates primer melting temperature. PCR product size and corresponding NCBI reference sequences are shown.