Table 2.
Twenty-one cataract genes mapped onto the canine genome
| Gene | Chromosome | Closest markers | LOD | θ | STS location | Canine ortholog location |
|---|---|---|---|---|---|---|
| BFSP2 | CFA23 | REN156G20 | 22.448 | 0.086 | 33427673–33428089 | 33388029–33450927 |
| REN210D03 | 17.827 | 0.169 | ||||
| CX46 | CFA25 | EST18C6 | 9.688 | 0.276 | 20988791–20989147 | 20986634–20987933 |
| BAC_372_A23 | 9.617 | 0.261 | ||||
| CX50 | CFA17 | CFOR12C10 | 20.47 | 0.056 | 61506499–61506884 | 61498923–61505209 |
| PEZ8 | 19.834 | 0.058 | ||||
| LIM2 | CFA1 | KLK2 | 18.201 | 0.035 | 108473188–108473537 | 108472780–08479290 |
| REN06N11 | 17.642 | 0.066 | ||||
| MIP | CFA10 | FH2537 | 25.039 | 0.09 | 3684287–3684594 | 3684852–3689232 |
| ATP5B | 21.428 | 0.132 | ||||
| SPARC | CFA4 | FH4018 | 14.097 | 0.048 | 60879945–60880285 | 60873070–60887224 |
| BAC_374_E21 | 12.897 | 0.093 | ||||
| CRYBB2 | CFA26 | CRYBB1 | 25.249 | 0.043 | 22335395–22335748 | 22331283–22338858 |
| REN131L06 | 24.11 | 0.064 | ||||
| CRYGA | CFA37 | AHT133 | 20.482 | 0.092 | 19453604–19453950 | 19453388–19455353 |
| REN105M20 | 20.482 | 0.092 | ||||
| CRYGB | CFA37 | BAC_381_J22 | 15.426 | 0.123 | 20124535–20124930 | 19440507–19442393 |
| FH2708 | 13.738 | 0.157 | ||||
| CRYGC | CFA37 | BAC_381_J22 | 24.954 | 0 | 19431676–19432046 | 19431504–19433284 |
| EST22D1 | 23.468 | 0.026 | ||||
| CRYGD | CFA37 | CRYGB | 15.027 | 0.045 | 19425779–19426157 | 19424588–19426264 |
| BAC_374_E1 | 8.03 | 0.354 | ||||
| CRYGS | CFA34 | BAC_374_K23 | 27.708 | 0.021 | 22165843–22166230 | 22165569–22166681 |
| REN266K05 | 27.283 | 0.022 | ||||
| CHX10 | CFA8 | EST10D4 | 23.799 | 0.025 | 50510969–50511356 | 50492693–50511026 |
| EST27E3 | 21.735 | 0.051 | ||||
| EYA1 | CFA29 | STS246A16 | 19.01 | 0.119 | 23177126–23177509 | 23176722–23342854 |
| C29_188 | 18.473 | 0.138 | ||||
| FOXE3 | CFA15 | FH3888 | 23.211 | 0.046 | 16294587–16294926 | 16295972–16298254 |
| FH3886 | 22.806 | 0.047 | ||||
| HSF4 | CFA5 | AHTH201REN | 13.127 | 0.052 | 85205320–85205780 | 85204186–85208678 |
| REN122J03 | 11.927 | 0.1 | ||||
| MAF | CFA5 | FH3450 | 9.517 | 0.244 | 74976764–74977099 | 74970434–74976948 |
| FH3113 | 9.427 | 0.224 | ||||
| PAX6 | CFA18 | WT1 | 17.245 | 0.148 | 38687699–38688008 | 38671041–38693640 |
| FH3824 | 17.15 | 0.148 | ||||
| PITX3 | CFA28 | EST3H1 | 19.137 | 0.083 | 17763487–17763792 | 17761261–17772191 |
| COL17A1 | 18.606 | 0.085 | ||||
| SIX5 | CFA1 | BAC_375_N13 | 21.594 | 0 | 112730879–112731247 | 112726865–12731063 |
| FH2598 | 21.594 | 0 | ||||
| SOX1 | CFA22 | EST3G2 | 26.711 | 0.022 | 62864830–62865127 | 62861615–62864949 |
| BAC_376_O1 | 24.774 | 0.045 |
PCR primers (Table 1) from 21 cataract genes were used for radiation hybrid-mapping using an RH5000 panel. Multimap® two-point linkage analysis was used to identify the chromosome location and two closest markers [17], along with the corresponding LOD score, and θ value (distance). To confirm these locations, PCR amplicons were analyzed against the 7.5X canine draft sequence to obtain sequence-tagged sites (STS) locations, which were compared with the corresponding canine ortholog locations within the draft sequence.