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Comparison of the effects of the IκB hybrids on NF-κB DNA binding. (A) Shown is an electrophoretic mobility-shift assay experiment performed with a constant amount of recombinant p65 in the presence or absence (lane 1) of the indicated recombinant IκB proteins. The molar ratio between p65 and IκB proteins was 1:2, 1:6, 1:18, and 1:54. (B) Shown is a Coomassie blue-stained SDS/PAGE gel displaying the purified IκB proteins used in this study.
