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. 1999 Jan 5;96(1):49–54. doi: 10.1073/pnas.96.1.49

Figure 5.

Figure 5

Comparison of the effects of different IκB proteins in cytoplasmic sequestration of NF-κB. P19 cells were transfected with a constant amounts of p65 (3 μg) along with the indicated IκB expression vectors (17 μg). Nuclear and cytoplasmic extracts were prepared and immunoblotted with a p65 antibody. The bottom of the figure indicates the transcriptional activity obtained from the cotransfected PRDII4CAT reporter plasmid.