Table 1.
PCR primers used to amplify genomic and cDNA segments of Hsf4
| Target | Primer name | Primer sequence (5′–3′) | Annealing temperature (°C) |
|---|---|---|---|
| HSF4 exon 1 | HSF4 1 F | CACTTTCGCGGCTTTGAC | 60 |
| HSF4 1.1 R | TCTCCCCATCTTTGCTTCAC | ||
| HSF4 intron 1 | HSF4 Intron 1 F | CCTCAACTCCTTCAGTGGTC | 60 |
| HSF4 Intron 1 R | TCACTTACGAGGAAGCTGGT | ||
| HSF4 exons 2–4 | HSF4 2 F | TGGACCCCCACAGTGAGTAT | 65 |
| HSF4 2 R | GAGGCTCCAACCAAAGAGC | ||
| HSF4 exons 5–6 | HSF4 3.1 F | GTATCCCCTTGCTGGCAAC | 63 |
| HSF4 3 R | CTGGAGGGTCTTTGGATGTG | ||
| HSF4 intron 6 | HSF4 Intron 6 F | GTGGTGTTGACACCTGCAT | 60 |
| HSF4 Intron 6 R | CATTGAATTTGGCAGATGCT | ||
| HSF4 exon 7 | HSF4 4.1 F | TTCCACTCCAGCATGTGACT | 62 |
| HSF4 4.1 R | CTAGGTCTGGGTGGTCGAGT | ||
| HSF4 intron 7 | HSF4 Intron 7 F | CCTCCAGGACCCCTACTTTA | 60 |
| HSF4 Intron 7 R | CCTGTGTCCTTCAGGAGATG | ||
| HSF4 exon 8 | HSF4 5 F | CCCAGCACAGATCATTTTCA | 60 |
| HSF4 5 R | TGCACCAGAGAAAAGCTTCA | ||
| HSF4 intron 8 | HSF4 Intron 8 F | CTGAAGGACACAGGCTTTCT | 60 |
| HSF4 Intron 8 R | CTGTAGCTCCCTTTCCCTTC | ||
| HSF4 exon 9 | HSF4 6 F | AAAGAAGAGCCGGCCAGT | 62 |
| HSF4 6 R | AGCTGCCTCAGGACCATAAA | ||
| HSF4 intron 9 | HSF4 Intron 9 F | TGTACAACAGCCTGAACCAA | 60 |
| HSF4 Intron 9 R | TGCCTCAGTCTCCCAAGTAG | ||
| HSF4 intron 9 | HSF4 Mut 1 F | TGACTTCTCAGGGTATTCTCCAA | 60 |
| HSF4 Mut 1 R | TACATGGGCTTTAGGGGTTG | ||
| HSF4 intron 9 | HSF4 Mut 2 F | GATCTGGCAGGATGGTTCAT | 60 |
| HSF4 Mut 2 R | AGCCACCCTTCCTCTTTGTT | ||
| HSF4 intron 9 | HSF4 Mut 3 F | TAAATTAATTAAAAACAAAGAGGAAGG | 60 |
| HSF4 Mut 3 R | TGCTCTTGTCTTAACCCACTG | ||
| HSF4 intron 9 | HSF4 Mut 3.1 F | CAGTGGGTTAAGACAAGAGCA | 60 |
| HSF4 7.1 R | GCTCTGCTTCATCCGTCTCT | ||
| Intron 9 insertion | LR HSF4 1 F | CCCCTTTAGCACTCAGTGGGTTAAGACAAG | 62 |
| LR HSF4 1 R | GTCCTACCCCAGGCAAAGAGATACTCACT | ||
| HSF4 exons 10–12 | HSF4 EX10 F | CAATAGCAGTTCTTTATGCA | 55 |
| HSF4 EX10 R | ATTCAGACCGTGATGGCTTC | ||
| HSF4 intron 12 | HSF4 Intron 12 F | TGACGGTCAAGGAGTTGAAT | 60 |
| HSF4 Intron 12 R | GCCTGGACATCTAGCATGAG | ||
| HSF4 exon 13 | HSF4 7.6 F | GGAACTCTGGGCCTGGAT | 60 |
| (RT) HSF4 3 R | GGCTTTTTCAGAGGGATGCAG | ||
| HSF4 upstream | HSF4 PROM 1 F | TCCGTCCCCTCTGTACACTC | 60 |
| HSF4 PROM 1 R | GGGCTCGGAAAGTCCTAGTT | ||
| HSF4 5′ cDNA | (RT) HSF4 1 F | CTTCCTCGGCAAGCTATGG | 60 |
| (RT) HSF4 1 R | TTGGCTCCTGTACTGCTG | ||
| HSF4 mid-cDNA | (RT) HSF4 8.1 F | GAGTTTCAGCATCCGAGCTT | 55 |
| (RT) HSF4 9.1 R | CTGTAGCTCCCTTTCCCTTC | ||
| HSF4 3′ cDNA | (RT) HSF4 7.5 F | GAGAGTCTGCTACCCCCAAT | 58 |
| HSF4-ET chimeric cDNA | HSF4 Intron 12 R | GCCTGGACATCTAGCATGAG | |
| Splice 1.1 F | GAAGGGAAAGGGAGCTACAG | 58 | |
| Splice 1.1 R | TCTCTGCCATTCTTCAGGTC |