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. Author manuscript; available in PMC: 2006 Jul 17.
Published in final edited form as: Oncogene. 2005 Sep 29;24(43):6482–6491. doi: 10.1038/sj.onc.1208810

Figure 3.

Figure 3

Detachment induces the activation of NF-κB. (a) Phosphorylated IκBα becomes detectable as a result of detachment and total protein levels decline over the first 30 min. p-IκB = phosphorylated-IκBα. (b) The protracted time course reveals that protein levels of IκBα recover by about 1 h following detachment. Secondly, increasing amounts of p65 protein accumulate in the nucleus coincidental with increased phosphorylated IκBα. n-p65, nuclear p65; c-p65, cytoplasmic p65. (c) IEC-18 were infected with adenovirus expressing NF-κB-promoted luciferase prior to detachment, then detached and harvested immediately or after 4 h, and luciferase activity was measured. *P < 0.01