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. Author manuscript; available in PMC: 2006 Jul 17.
Published in final edited form as: Oncogene. 2005 Sep 29;24(43):6482–6491. doi: 10.1038/sj.onc.1208810

Figure 6.

Figure 6

IKKα becomes phosphorylated as a function of detachment. (a) IKK rapidly becomes phosphorylated following detachment, without a discernable change in total IKK protein. p- IKK: phosphorylated IKK. (b) Incubation of cells in PAO (0.5 μm) but not gliotoxin (0.5 μg/ml) prior to detachment results in less phosphorylated IKKα after detachment. (c) Preincubation of cells in PAO results in a rapid and specific decline of total IKKα protein in detached cells. The attached control cells were harvested after 60 min incubation in PAO. Dtd: detached