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. Author manuscript; available in PMC: 2006 Jul 17.
Published in final edited form as: Oncogene. 2005 Sep 29;24(43):6482–6491. doi: 10.1038/sj.onc.1208810

Figure 7.

Figure 7

Enhanced NF-κB activation fails to increase the numbers of IEC-18 cells surviving detached. (a) Infection of cells with adenovirus overexpressing native NIK protein for 24 h prior to detachment results in greater levels of phospho-IκBα than detached cells harvested at 1 h. NIK-overexpressing cells show greater levels of nuclear NF-κB (b) and less activation of caspase 3 (c) than uninfected cells or cells infected with virus expressing GFP and then detached for 24 h. (d) NIK-overexpressing detached cells do not survive in greater numbers than detached uninfected cells, measured as DiOC6 staining after 24 h. The number of cells surviving expressing IκBAA, included for comparison, was significantly reduced, *P < 0.05