Figure 1.

Induction of colitis by indomethacin in mice treated with 3% DSS and suppression of this effect by dm-PGE₂. (a–c) Wild-type C57BL/6 mice were treated with either 3% DSS alone (diamonds), 3% DSS and indomethacin (squares), or 3% DSS, indomethacin, and dm-PGE₂ (triangles), and monitored for body weight loss (a), diarrhea (b), and hemoccult (c) for 7 days. (d and e) On day 7, the mice were sacrificed. The colon was dissected for histological analysis with H&E staining (e), and the histological injury scores were obtained (d). Blood was collected for measurement of the WBC number (f) and HCT (g). The weights of spleens of the sacrificed animals are shown (h). N, mice treated with vehicle; D, mice treated with 3% DSS alone; D+I, mice treated with 3% DSS and indomethacin; D+I+E, mice treated with 3% DSS, indomethacin, and dm-PGE₂. Data in a–d and f–h are means ± SEM from six animals. *P < 0.05 versus D (Dunnett). ^#P < 0.05 versus D+I (Dunnett multiple comparison test). Scale bars, 200 μm in e.