Figure 3.

Bile acids decrease apoA-I mRNA levels in primary human hepatocytes. (a and b) Primary human hepatocytes were incubated for 48 hours with CDCA (10 μM) or vehicle. (a) Human apoA-I mRNA was analyzed by Northern blot. 28S mRNA was measured as control. (b) Densitometric quantification of apoA-I/28S mRNA levels. Values are expressed as a percentage of mRNA levels in control cells and are means ± SD (n = 3). SHP mRNA levels were measured by quantitative RT-PCR and normalized to 28S RNA levels as control. Values are expressed as fold induction of mRNA levels in control cells and are means ± SD (n = 3).