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. 1999 Jan 5;96(1):67–72. doi: 10.1073/pnas.96.1.67

Figure 1.

Figure 1

Transcription assays of wild-type and Δpgd1 holoenzymes. Transcription was performed with highly purified transcription factors and DNA templates containing binding sites for Gcn4 (GCN4:G−) and Gal4 (GAL4:G−). Gal4–VP16 activation (31-fold for wild-type holoenzyme, 1.8-fold for Δpgd1) was quantitated by comparing transcription in the presence and absence of the activator on the GAL4:G− template and dividing the ratio by any change in transcription of GCN4:G− template. Gcn4 activation (8.2-fold for wild-type holoenzyme, 6.9-fold for Δpgd1) was measured with the GCN4:G− template in a similar manner.