Table 2.
Gal4-VP16 activation in wild-type, Δmed2, and Δsin4 strains
| Strain | Vector | Gal4–VP16 | Gal4–VP16FA |
|---|---|---|---|
| MED2 wild type (MG106) | <1 | 1156 | 568 |
| Δmed2 (MG107) | <1 | 138 | 35 |
| SIN4 wild type (DY1880) | <1 | 2806 | 660 |
| Δsin4 (DY1876) | <1 | 1763 | 342 |
Levels of β-galactosidase activity were assayed in strains with an ARS-CEN plasmid containing Gal4–VP16 or Gal4–VP16 bearing the Phe-442 to Ala mutation (Gal4–VP16FA) under control of the ADH1 promoter, and the pLGSD5 GAL (lacZ) reporter plasmid (23). The units of activity are normalized to cell OD600 and results shown are the means from at least three replicate assays.