Figure 1.

Colony formation by mononuclear bone marrow cells from VDR KO and WT mice. Mononuclear cells were obtained from femoral bone marrow plugs and grown in methylcellulose media containing cytokines and various concentrations of 1,25(OH)₂D₃. Colonies were counted on day 10 of culture. Control cultures from WT and KO mice contained a mean of 96 ± 37 and 98 ± 15 myeloid colonies, respectively. Numbers represent the mean ± SD of three experiments performed on triplicate wells using cells from three KO and three WT mice. Triangles, granulocyte colonies; circles, macrophage colonies; squares, mixed granulocyte/macrophage colonies.