Figure 5.

Cytokine production by splenocytes stimulated to differentiate toward either Th1 or Th2 cells. Spleen cells were stimulated as described in Figure 4 legend, but with the addition of a cytokine or antibody that enhances Th1 or Th2 differentiation. (a) Exogenous IL-12 (50 μg/ml) was added to induce Th1 cell differentiation. (b) Anti–IL-12 (100 μg/ml) was added to induce Th2 cell differentiation. Conditioned media were analyzed for cytokine content by ELISA. The results represent the mean ± SD of three experiments performed on triplicate wells using cells from three KO and three WT mice.