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. 2002 Apr 15;109(8):1111–1120. doi: 10.1172/JCI14255

Figure 7.

Figure 7

Reduced H-AChR– and α320-337 peptide–specific lymphocyte proliferative response and IFN-γ and IL-2 secretion in DQ6 mice compared with DQ8, DR3, and DQ8×DR3 F1 mice. At termination of the long-term experiment (day 90), LNCs from DQ6, DQ8, DR3, and DQ8×DR3 F1 mice were cultured in vitro with H-AChR (0.5 μg/ml), peptide α320-337 (40 μg /ml), and PBS, and supernatants collected after 48, 72, and 96 hours were used for IL-2, IL-10, and IFN-γ measurement, respectively. Because of the reduced number of cells, the LNCs from two mice in one group were pooled. For the lymphocyte proliferation, total samples were six DQ6 mice and eight each of DQ8, DR3, and DQ8×DR3 F1 mice; for the supernatant cytokine test, total samples were three DQ6, seven DQ8, five DR3, and five DQ8×DR3 F1 mice. (a) Lymphocyte proliferation is measured in Δcpm (cpm with antigen minus cpm with medium). Mean cpm with medium was: DQ6, 6363; DQ8, 6218; DR3, 6795; DQ8×DR3 F1, 7567. Δcpm for T-AChR was: DQ6, 2152; DQ8, 7213; DR3, 9703; DQ8×DR3 F1, 1046.4. (b and c) Suppressed production of AChR-specific IFN-γ (b) and IL-2 (c) in DQ6 mice compared with DQ8, DR3, and DQ8×DR3 F1 mice. *P < 0.05, Student’s t test. The error bars are SE. Mean values (pg/ml) with medium for IFN-γ were: DQ6, 21.67; DQ8, 22.19; DR3, 36.07; DQ8×DR3 F1, 26.59; and for IL-2 were: DQ6, 27; DQ8, 54.18; DR3, 47.42; DQ8×DR3 F1, 574. (d) IL-10 secretion in supernatant of each strain of transgenic mice. Mean values (pg/ml) with medium were: DQ6, 27; DQ8, 21.4; DR3, 28; DQ8×DR3 F1, 21.79.