Figure 2.

Summary maps of DnaB and RepA that were used in the RepA–DnaB interaction experiments. (A) The ORF of dnaB showing the nucleotide coordinates at the top. The coordinate 1 is the first residue of the ATG initiator codon. The coordinates of the two N-terminal peptides AgeI and BC2 are shown in nucleotides and within brackets in codon numbers (e.g., c1-c208 for AgeI). The location of the m₃ double mutant D253A, K254A are shown. The + and − symbols indicate positive and negative protein–protein interaction, respectively, between immobilized dnaB and RepA in solution. (B) The ORF of RepA is shown with nucleotide coordinates at the top. The nucleotide 1 corresponds to the first residue of the ATG initiator. The N-terminal and C-terminal peptides are identified by nucleotide coordinates and by codon number designation in parenthesis. The + and − symbols mark the peptides showing positive and negative interaction, respectively, with DnaB. The m_a quadruple mutant of RepA (K124A, K125A, K128A, and Y129A) and the nucleotide substitutions that were used to generate the mutant are shown.