Figure 2.

Efficiency of lipofection-mediated intracellular neutralization by 7D9 at different time points after infection. MA104 cells were infected with TLPs of RRV. At indicated time points after infection, 7D9 mAb was transfected into cells (0.025 mg/ml) using lipofectin. Viral antigen in the cells was detected 10 hours after initial RRV infection by immunostaining. Data are expressed as percentage of focus reduction as compared with control. *Focus reduction was below 50% and significantly different from that at time 0 (P < 0.05). Results were calculated from triplicate measurements and represent two separate experiments.