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. 1999 Jan 5;96(1):79–84. doi: 10.1073/pnas.96.1.79

Figure 4.

Figure 4

sAC is a single copy gene preferentially expressed in testis. (A) Southern blots of 10 μg rat genomic DNA digested with BamHI (B), EcoRI (E), HindIII (H), and XhoI (X) probed at high (Left) or low (Right) stringency by using the 1-kb PCR-generated sAC fragment containing both presumptive catalytic domains. (B) Rat multiple tissue Northern blot (CLONTECH) representing approximately 2 μg poly-A+ RNA from testis (T), kidney (K), skeletal muscle (Sk), liver (Li), lung (Lu), spleen (S), brain (B), and heart (H) probed with 1-kb PCR-generated sAC fragment (Upper) or with actin control (Lower). The sAC transcript is approximately 5.3 kb and, in most tissues, actin is approximately 2.0 kb.