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Peptidyl transferase activity of partially deproteinized large ribosomal subunits. 35S-labeled T. aquaticus 50S subunits were treated with proteinase K in the presence of SDS (proteinase K/SDS) followed by one, two, three, four, or five phenol extractions. Peptidyl transferase activity of the material precipitated from the aqueous phase with ethanol (y axis) was plotted against the amount of remaining proteins (x axis). Activity of the untreated 50S subunits (50S) was taken as 100%.
