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Extraction of T. aquaticus 30S and 50S ribosomal subunits with SDS. Ribosomal subunits were incubated in the absence (C) or in the presence (SDS) of 0.5% SDS as described in Materials and Methods, purified by sucrose gradient centrifugation, and analyzed by SDS/urea gel electrophoresis. Positions of molecular weight markers are indicated.
