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. 1999 Jan 5;96(1):91–96. doi: 10.1073/pnas.96.1.91

Figure 3.

Figure 3

(A) Synthesis of oligonucleotide-NLS. PAGE analysis of the formation of the oligonucleotide-peptide conjugate is shown. Aliquots of the reaction mixture were taken at different times and analyzed on a 20% denaturing gel after radiolabeling of the oligonucleotides. Lanes: 1, oligonucleotide-NH2; 2, oligonucleotide-NH2/SMCC reaction mixture after 2 h; 3–5, reaction mixture 1.5 h, 3 h or overnight after addition of the NLS-peptide to the oligonucleotide-NH2/SMCC mixture, respectively. (B) The presumed oligonucleotide-NLS conjugate is a substrate of proteinase K. Radiolabeled oligonucleotide-NH2 or the crude oligonucleotide-NLS reaction mixture were digested with proteinase K. Products were analyzed on a 20% denaturing gel and show total conversion of oligonucleotide-NLS into a faster migrating band, presumably oligo-Mal-Cys.