Table 1.

Free DNA ends decrease efficacy

DNA form	Symbol	Transfection efficiency, RLU/mg of protein
Uncapped CMVLuc		1 ± 0.4 × 105
Hemi-capped CMVLuc		9 ± 2 × 104
CMVLuc		2.5 ± 0.6 × 106
Circular pCMVLuc plasmid		2.5 ± 0.2 × 108 3 ± 1 × 104*

BNL CL.2 hepatocytes were transfected with 200 ng of DNA per well. Complexes were formed with 25-kDa PEI (N/P = 10) in 150 mM NaCl and in the absence of serum. RLU, relative light units. 

^*Transfection with 20 ng of plasmid.